| # | Title | Artist | Rating | Length |
|---|
| 1 | Shortnin' / Henduck | Otha Turner and The Rising Star Fife and Drum Band | | 2:52 |
| 2 | Long John | Lightnin' Washington & Work Group | | 5:25 |
| 3 | Crazy Blues | Mamie Smith | | 3:29 |
| 4 | St. Louis Blues | William Christopher Handy | | 3:02 |
| 5 | Muddy Water | Bessie Smith | | 3:08 |
| 6 | Match Box Blues | Blind Lemon Jefferson | | 3:01 |
| 7 | Billy Lyons & Stack-O-Lee | Furry Lewis | 5 | 2:36 |
| 8 | Ma Rainey's Black Bottom | Ma Rainey | 1 | 3:03 |
| 9 | Dark Was the Night, Cold Was the Ground | Blind Willie Johnson | 3.75 | 3:21 |
| 10 | Savoy Blues | Louis Armstrong | | 3:27 |
| 11 | Downtown Blues | Frank Stokes | | 3:10 |
| 12 | Frankie | Mississippi John Hurt | 3.75 | 3:24 |
| 13 | Fishing Blues | Henry Thomas | 5 | 2:43 |
| 14 | How Long, How Long Blues | Leroy Carr | 3.5 | 3:06 |
| 15 | Canned Heat Blues | Tommy Johnson | 5 | 3:38 |
| 16 | Statesboro Blues | Blind Willie McTell | | 2:30 |
| 17 | It's Tight Like That | Tampa Red & Georgia Tom | | 3:04 |
| 18 | Pine Top's Boogie Woogi | Clarence “Pine Top” Smith | | 3:22 |
| 19 | Guitar Blues | Lonnie Johnson | 3 | 3:15 |
| 20 | Pony Blues | Charley Patton | | 2:59 |
| 21 | Diddie Wah Diddie | Blind Blake | 3 | 2:58 |
| 22 | K.C. Moan | Memphis Jug Band | 4 | 2:32 |
| 23 | Standing on the Corner (Blue Yodel No. 9) | Jimmie Rodgers | 3 | 2:39 |
| 24 | Sittin' on Top of the World | Mississippi Sheiks | 3 | 3:04 |
| 25 | Preachin' the Blues | Son House | | 3:02 |