| 1 | Gospel Church, Harlem / Thank You, Lord | 4:30 |
| 2 | Reverend Gary Davis / If I Had My Way | 4:47 |
| 3 | Yvonne Hunter / Have You Ever Been Mistreated | 1:39 |
| 4 | Muddy Waters / I Can't Be Satisfied | 2:44 |
| 5 | Bob Dylan / Roll On John | 3:27 |
| 6 | Roscoe Holcomb / Man Of Constant Sorrow | 2:59 |
| 7 | Doc Watson and Gaither Carlton / Hicks Farewell | 4:31 |
| 8 | Carter Stanley / Come All You Tenderhearted | 3:38 |
| 9 | Mary Townsley / Young But Growing | 3:30 |
| 10 | Alice Gerard and Hazel Dickens / TB Blues | 3:29 |
| 11 | Bill Monroe / John Henry | 1:35 |
| 12 | Eck Robertson / Sally Goodin' | 3:42 |
| 13 | Sidna Myers / Twin Sisters | 0:57 |
| 14 | Wade Ward and Charlie Higgins / Sally Johnson | 2:15 |
| 15 | David Amram Quartet / Pull My Daisy | 4:34 |
| 16 | Rufus Cohen and Wade Patterson / So Long Go | 3:03 |
| 17 | Last Forever / Who'll Water My Flowers? | 2:37 |
| 18 | Elizabeth Cotten / Oh Babe, It Ain't No Lie | 2:08 |
| 19 | Woodie Guthrie / Ramblin' Round | 2:18 |
| 20 | Alan Lomax / Love My Darling-O | 1:57 |
| 21 | New Lost City Ramblers / Buck Creek Girls | 3:02 |
| 22 | Huayno stringband / Paloma Blanca | 2:21 |
| 23 | Sweet's Mill Band / Kitchen Girl | 2:42 |
